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Abstract

A SIMPLE SPECTROPHOTOMETRIC ASSAY OF LAMIVUDINE IN PHARMACEUTICAL FORMULATIONS

G. Srihari, K. Prabhavthi, N. Umamaheswar Reddy and N. Rami Reddy*

ABSTRACT

A simple, sensitive and accurate spectrophotometric method has been developed for the estimation of lamivudine in bulk and pharmaceutical dosage forms. The amino group in lamivudine is diazotised with sodium nitrite and hydrochloric acid at 0oC temperature. After diazotisation, the diazonium salt is coupled with resorcinol. The orange red coloured chromogen formed in the method is stable for more than 24 hours. The orange red coloured chromogen is measured at the wavelength of maximum absorbance 460 nm against the reagent blank. The results obtained with the proposed method are in good agreement with labeled amounts, when marketed pharmaceutical preparations are analyzed.

Keywords: Spectrophotometry, resorcinol, lamivudine, Pharmaceutical and Formulation.


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