WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

THIN LAYER CHROMATOGRAPHIC ANALYSIS AND EVALUATION OF EFFECT OF MACERATION AND REFLUXING TIME ON ANTIPLASMODIAL ACTIVITY OF GUIERA SENEGALENSIS ROOTS

Enas Ali Hassan Alkangar*1, Matab Amgad Omer2, Tahani Abdallah Dafaalla2, Mahasen Gaiballa Mohamedain2, Shimaa Almamoon Kabashi2 and Fatima Ahmed Mubarak2

ABSTRACT

This study aimed to determine the extraction method and solvent of the
highest antimalarial components extracting capacity among the
methods and solvents used and to identify the effect of maceration and
refluxing time on Anti-plasmodial activity of the root of Guiera
senegalensis. The roots was extracted by maceration for different times
with petroleum ether, Chloroform (24, 48 and 72 hour) and ethanol
70% (6, 24, 48 hour) and refluxing methods for different times by
Chloroform (15, 30, 60 min.), yield % was calculated. Phytochemical
screening and thin layer chromatographic analysis of all 12 extracts
were carried out using standard methods. All extracts evaluated for
Antiplasmodial activities against Plasmodium falciparum. The highest
yield % (2.36%) was observed with ethanol 70 % (24h) and the lowest one (0.10%) was
observed with chloroform (48h), phytochemical analysis exhibited the presence of Essential
oils, Coumarins, Anthraquinones, Alkaloids, Saponins, Terpenoids, Tannins, Flavenoids and
Cardiac glycosides. Ether extract (24h) and all chloroform extracts resulted in parasite
survival of less than 50 %, the highest activity observed with chloroform extract (24hour)
with IC50 (113.24 μg/ml). Ether extracts (48 and 72 h) in addition to all ethanol extracts were
found to be inactive. The antiplasmodial activity was found to be affected adversely by long
extraction time. Temp. has been found to have negative effect on the antiplasmodial activity
of Guiera senegalensis.

Keywords: Guiera senegalensis, Antiplasmodial, Thin layer chromatography, Maceration, Reflux.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More