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Abstract

SIMULTANEOUS ESTIMATION OF RISPERIDONE AND TRIHEXYPHENIDYL HYDROCHLORIDE BY USING ISOCRATIC REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD

S. B. Kulkarni, R. S. Lokhande, P.V. Jadhav, S. P. Janwadkar, P. K. Rana*, D. K. Yadav

ABSTRACT

A simple, precise, fast Reversed phase High Performance Liquid Chromatographic (RP-HPLC) assay method by external standard method was developed for the determination of Risperidone and Trihexyphenidyl HCl in a solid dosage form. The chromatographic separation was performed using Phenomenex Bondalone 300 x 3.90 mm, 10 μL column. Analyses were resolved by using mobile phase 40:60 mixture of Ammonium acetate pH4.9: Acetonitrile at UV 220 nm as detection wavelength.

Keywords: RF-HPLC, simultaneous estimation, risperidone, trihexyphenidyl hydrochloride, isocratic


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