Abstract

BIOFILM FORMATION BY Bacteroides fragilis ISOLATED FROM WOMEN WITH BACTERIAL VAGINOSIS

Prof. Dr. Ilham A. Bunyan*, Prof. Dr. Bushra J. Umran and Zahraa Kais Salman

ABSTRACT

Many novel, fastidious and uncultivated bacterial species are related with Bacterial vaginosis that is one of the most common genital infections among women in the childbearing age. These are called bacterial vaginosis associated bacteria (BVAB), present in trace amount and have a significant role in the infection. Biofilm forming by these bacteria led to increase chance of bacterial vaginosis incidence due to increase bacterial resistant. A total of 300 high vaginal swabs were obtained from 150 non-pregnant women with bacterial vaginosis depending on Amsel criteria. Two swabs where taken for each patient, these swabs were cultivated anaerobically on selective media, Bacteroides Bile Esculin agar for Bacteroides fragilis isolation. Samples were collected from patients those admitted to the out-patient clinics of Gynecology and Obstetrics in Babylon Maternity and Pediatrics teaching hospital and Al-Hillah General Teaching Hospital. Cultivated swabs were gave 31(21%) positive results for Gram negative, anaerobic Bacteroides fragilis.

Keywords: Biofilm, Bacteroides fragilis, Bacterial vaginosis, Anaerobic conditions.