BIOFILM FORMATION BY Bacteroides fragilis ISOLATED FROM WOMEN WITH BACTERIAL VAGINOSIS
Prof. Dr. Ilham A. Bunyan*, Prof. Dr. Bushra J. Umran and Zahraa Kais Salman
ABSTRACT
Many novel, fastidious and uncultivated bacterial species are related
with Bacterial vaginosis that is one of the most common genital
infections among women in the childbearing age. These are called
bacterial vaginosis associated bacteria (BVAB), present in trace
amount and have a significant role in the infection. Biofilm forming by
these bacteria led to increase chance of bacterial vaginosis incidence
due to increase bacterial resistant. A total of 300 high vaginal swabs
were obtained from 150 non-pregnant women with bacterial vaginosis
depending on Amsel criteria. Two swabs where taken for each patient,
these swabs were cultivated anaerobically on selective media,
Bacteroides Bile Esculin agar for Bacteroides fragilis isolation. Samples were collected from
patients those admitted to the out-patient clinics of Gynecology and Obstetrics in Babylon
Maternity and Pediatrics teaching hospital and Al-Hillah General Teaching Hospital.
Cultivated swabs were gave 31(21%) positive results for Gram negative, anaerobic
Bacteroides fragilis.
Keywords: Biofilm, Bacteroides fragilis, Bacterial vaginosis, Anaerobic conditions.
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