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Abstract

ANTIBACTERIAL ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES FROM VIOLA SERPENS AGAINST MULTI DRUG RESISTANT STRAINS OF STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA

Anu Kumar*, Rita Singh Mazumdar and Tejpal Dhewa

ABSTRACT

Background: Antibiotic resistance is a major concern at present. Pathogenic microorganisms are becoming resistant to drugs day by day. Objectives: The present work aims to study the MAR (Multiple Antibiotic Resistance) index and to evaluate the antibacterial efficacy of biosynthesized silver nanoparticles from leaves of Viola serpens against multidrug-resistant clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa isolated from different sources such as burn patients, wounds, blood, nasal and pus samples. Materials and Method: In the present work, we made efforts to study the synergistic effects of medicinal values of Viola serpens and nanosciences with the field of biomedicine for the development of a safer alternative to allopathic drugs against these MDR strains. Further antibacterial potentiality of biologically synthesized silver nanoparticles was evaluated against multidrug resistant strains by using agar well diffusion assay. Results and Discussions: Biosynthesized silver nanoparticles showed the zone of inhibition ranges from 11mm to 18mm. The Synergistic activity of AgNPs and leaves extract was found to be more potent (<18mm Zone of inhibition) against MDR strains. Conclusion: In conclusion, biosynthesized Silver nanoparticles from Viola serpens showed enhanced antibacterial potential which makes them a potent source of antibacterial agent and it can increase the effectiveness of drugs.

Keywords: Viola serpens, Silver nanoparticles, Antibiotic resistance, Antibacterial activity.


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