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Abstract

HPTLC AND RP-HPLC METHODS FOR THE ESTIMATION OF MANGIFERIN IN MANGIFERA INDICA EXTRACT AND ITS FORMULATION

Jayaprakasam. R*, T.K. Ravi

ABSTRACT

Two simple and accurate HPTLC and HPLC methods were developed and validated for the estimation of mangiferin in Mangifera indica (Anacardiaceae) and in its formulation using standard marker. Oxitard capsule was selected for the current study. The validated HPTLC analysis was carried out on plant extracts and formulation using ethyl acetate : methanol : formic acid : water (10:1:1:0.5%v/v/v/v) as mobile phase and the detection wavelength was 270nm. The Rf values of the extract and formulation were compared with that of the standard. The standardization of the extract and formulation were carried out by validated HPLC method . The optimized solvent system was Methanol: orthophosphoric acid (65:134%v/v). The flow rate was 1ml/minute. The detection wavelength was 258nm.The plant extract was standardized, quantified and validated by newly developed HPTLC and HPLC methods. The estimation of marker constituent present in the extract and formulation can be utilized in routine estimation and standardization of medicinal plant extract.

Keywords: Mangifera indica, Mangiferin, Oxitard capsule, HPTLC, RP-HPLC.


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