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Abstract

PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL ACTIVITY OF ALLIUM TUBEROSUM

Angayarkanni T.*, Dhivya R. and Santny A. S.

ABSTRACT

Medicinal plants are excellent sources of information for a wide variety of chemical constituents which could be developed as drugs with precise selectivity. These are the reservoirs of potentially useful chemical compound which could be serves as a newer lead and clues for modern drug design. Allium tuberosum has a wide variety of antimicrobial properties which is a fine source in selecting the plant and describing its activity. The method of extraction and methods opted for describing its antimicrobial activity has been done by the use of ethanolic extractions for antimicrobial activity, NCCS method for antibacterial activity and antifungal activity by PDA media. The results obtained shows that B.subtilis shows maximum inhibition when compared with other microorganisms. Antifungal activity by PDA media proves that the Sclerotium rolfsii has shown maximum inhibition of 5mm with Nystatin as control. Zone of inhibition of leaves of Allium tuberosum has minimum formation of zone at various concentrations such as 10μl, 20μl, 30μl, 40μl using organism namely Aspergillus flavus, Fusarium verticillodies, Sclerotium rolfsii. It has been shown that Fusarium verticillodies shows maximum inhibition of 14mm with 40 μl of A.tuberosum concentration. The ethanolic extract of leaves of Allium tuberosum was inoculated against K.pneumoniae, P.aeruginosa, S.aureus and B.subtilis in the concentration of 8-0.25 mg/100μl. The growth of S.aureus, P.aeruginosa, K.pneumoniae and B.subtilis were inhibited by ethanolic extract of Allium tuberosum of 8-1.0 mg/100μl concentration. S.aureus and P.aeruginosa was found to have significant killing effect at 8-1.0 mg/100μl whereas K.pneumoniae has the killing effect at 8-2 mg/100μl concentration. B.subtilis found to have killing effect at 8-0.5 mg/100μl.

Keywords: Agar well diffusion method Nystatin Potato dextrose agar media. Reactive oxygen species.


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