WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF SITAGLIPTIN PHOSPHATE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

M. Phanisri*, M. Jhansi Lakshmi, D. Rama Brahma Reddy and H. Padmalatha

ABSTRACT

A simple and rapid reversed phase-high performance liquid chromatographic method was developed for simultaneous determination of Sitagliptin Phosphate In Bulk And Pharmaceutical Dosage forms. The elution was done with a mobile phase of Methanol, Acetonitrile & 0.1% ortho phosphoric acid in the ratio of 40:55:05 & adjust to pH 4.1 by using triethyl amine on Zodiac C18 column (250 × 4.6 mm, 5μ). The wavelength of detector was set at 265 nm. The reliability and analytical performance of the proposed HPLC procedure were statistically validated according to the respect of linearity, ranges, precision, accuracy, repeatability, reproducibility, detection and quantification limits. Linear ranges were established between 60-210 μg/mL for the drug. The LOD and LOQ for Sitagliptin was found to be 0.05, 0.16 respectively. The described High Performance Liquid Chromatography method was successfully employed for the analysis of pharmaceutical formulations.

Keywords: Sitagliptin, RP- HPLC, validation, UV Detection, Zodiac C18 column.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More