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Abstract

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF ACECLOFENAC AND THIOCOLCHICOSIDE IN BULK AND TABLETS DOSAGE FORMS

Venkata S Rao Somisetty*, Dr.D.Dhachinamoorthi,Rs S Lakshmikeerthana, Santhi Priya Bathula

ABSTRACT

A simple, selective, precise high-performance thin-layer chromatographic method for simultaneous determination of Aceclofenac and Thiocolchicoside in bulk and pharmaceutical combined dosage form was developed and validated. The method employed HPTLC aluminum plates precoated with silica gel 60F-254 (10×10) as the stationary phase. The solvent system consisted of Ethyl acetate: Methanol: 1% Glacial acetic acid (80:20:1% v/v). The system was found to give a compact spot for Aceclofenac (Rf = 0.41± 0.02) and Thiocolchicoside (Rf = 0.20±0.02). Densitometric analysis of aceclofenac and Thiocolchicoside was carried out in the absorbance mode at 276 nm. Linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9912 with respect to peak area in the concentration range 40-160 ng per spot for aceclofenac and r2 = 0.9976 with respect to peak area in the concentration range 40–160 ng per spot for Thiocolchicoside. The method was validated for precision, recovery and robustness. The limits of detection and quantitation were 20.00 and 40 ng per spot for aceclofenac and 10 and 20 ng per spot for thiocolcoside, respectively. Statistical analysis proved that the method is selective, precise and accurate for the estimation of aceclofenac and Thiocolchicoside.

Keywords: Aceclofenac, HPTLC, Thiocolcoside, pharmaceutical formulation.


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