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Abstract

ESTIMATION OF PHENOLIC CONTENT, FLAVONOID CONTENT, ANTIOXIDANT PROPERTYAND ALPHA AMYLASE INHIBITION ACTIVITY OF POMEGRENATE (PUNICAGRANATUM) AND KINNOW (HYBRID OF CITRUSNOBILIS AND CITRUS DELICIOSA) PEELS

Saxena P.* and Jain S.

ABSTRACT

The objective of this study was to assess the antioxidant properties of the peels of selected citrus fruits that are readily available in India. A relation was derived between the antioxidant activity and the alphaamylase inhibition activity to ascertain their use as a potential nutraceutical product thereby reducing solid kitchen waste. The fruit peel extracts of pomegranate (Punicagranatum) and Kinnow (Hybrid of Citrus nobilis and Citrus deliciosa) were prepared in water. Phenolic content, flavonoid content and free radical scavenging activity were estimated. The total phenolic content was found to be 22.86 mg of GAE /100 g for kinnow and 24.6 mg of GAE/100g for pomegranate. Total flavonoid content was found to be 580 mg of quercetin equivalent/100g for kinnow and 1163.34mg of quercetin equivalent/ 100g for pomegranate. In vitro antioxidant activity was found to be 122.75 mg of Ascorbic acid equivalent/100g for kinnow and 545.75 mg of Ascorbic acid equivalent/100 g for pomegranate. Free radical scavenging activity was calculated using DPPH and was found to be 34.6% for kinnow and 84.2% for pomegranate. Alpha amylase inhibition activity was calculated and IC50 value for kinnow was 4.9 mg/mL and for pomegranate was < 2mg/mL. It was inferred that both pomegranate and kinnow peels exhibit excellent antioxidant and alpha amylase inhibition activity and can be used as potential nutraceutical products to cure oxidative stress related diseases like diabetes.

Keywords: DPPH, alpha amylase, oxidative stress related disorders, free radicals, antioxidant.


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