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Abstract

ISOLATION, IDENTIFICATION AND SCREENING OF PATCHOULI OIL TOLERANT MICROORGANISMS FROM PATCHOULI RHIZOSPHERE AND PHYLLOPLANE

Ashwini Jayaram, Vasundhara M.*, Priyanka R. and Ananda S.

ABSTRACT

In this study we isolated, screened and identified patchouli oil tolerant microorganism from rhizosphere and phylloplane. Rhizosphere soil samples from patchouli growing plot and patchouli leaves were collected at University of Agricultural Sciences Bangalore. Five organisms out of 21 isolated were found to be tolerant in the initial screening step. These five organisms were identified as Botryosphaeria spp, Aspergillus terreus, Phoma spp., Fusarium solani complex and Penicillium citrinum. The organisms were tested for patchouli oil tolerance (0.1 to 0.4%). Varying level of tolerance was recorded, highest tolerance index was observed in Botryosphaeria spp (0.81 - 0.56) and lowest in Fusarium solani complex (0.45 to 0.19).

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