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Abstract

IN VITRO REGENERATION OF COMMELINA DIFFUSA BURM. F. USING NODAL EXPLANTS

A. V. P. Karthikeyan, I. Sudan* and R. Satheeshkumar

ABSTRACT

In Vitro Regeneration of plantlets has been achieved in an important
medicinal plant of Commelina Diffusa (Commelinaceae) through nodal
explants. The nodal explants were cultured on MS medium
supplemented with different concentrations of BAP (5 μM -25 μM)
and KIN (5 μM -25 μM) alone. Among the two cytokinins tested, 10
μM of BAP was found to induce more number of shoots (2.75 ± 0.47)
than 10 μM KIN (3.2 ± 0.41). A maximum number of shoots was
induced from all the various concentrations of BAP and KIN. The
highest frequency of 90% shoot induction was observed on MS basal
medium supplemented with 10μM BAP and 10 μM KIN alone. For
root induction regenerated shoots were transferred to the half strength MS basal medium
supplemented with different concentration of IBA and IAA. The maximum numbers of roots
(15.6 ± 1.48) were obtained from 6 μM IAA with mean root length of 6.7cm. The plantlets
were successfully transplanted to the paper cup for hardening and then it was transferred to
the field with 85% survival rate.

Keywords: Commelina Diffusa Burm. f., nodal explants, BAP, KIN, IAA, IBA, MS medium.


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