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Abstract

CALLUS PRODUCTION OF GLOBE ARTICHOKE AND MILK THISTLE: IN VITRO HYPOLIPIDEMIC AND ANTIOXIDANT ACTIVITIES

Shawky A. Bekheet, Mohamed K. El-Bahr, Sanaa A. Ali, Manal A. Hamed

ABSTRACT

The goals of this study were to develop an in vitro callus growth system of globe artichoke and milk thistle plants and investigate their hypolipidemic and antioxidant activities. For callus induction, leaf explants were cultured on MS medium supplemented with 5 mg/l NAA + 2 mg/l Kin + 0.1 mg/l GA3. For solidifying culture medium, gelrite showed high growth of callus in comparison to agar. The effect of picloram added to culture medium on development and growth of callus was examined. Addition of 3 mg/l picloram to culture medium registered the best results of calli growth of the two plants presented as fresh weight and growth value. The influence of salicylic acid (SA) on growth parameters of calli of the two plants was investigated. The most effective level of SA was 75 μM in which the highest callus fresh weight and dry matter were registered. Otherwise, effect of the different concentrations of jasmonic acid (JA) on fresh mass and dry matter of both two plants species was tested. Among the tested concentrations of JA, 50 μM was more suitable for callus growth. However, it was noticed that, callus of milk thistle was more positive responding for exogenous application of both SA and JA compared with callus of globe artichoke. In vitro hypolipidemic effects of extracts of globe artichokes and Milk thistle callus cultures were studied. Extracts of calli of both plants showed hypolipidemic viability effects in a dose-dependent manner. In addition, globe artichoke and milk thistle extracts recorded potent antioxidant effects as compared to their standard. There were significant differences between extracts of calli and standards at the low levels groups (0.01, 0.1, 1.0 mg/l). Moreover, globe artichoke extract showed more potent hypolipidaemic and antioxidant effects than the milk thistles extract.

Keywords: globe artichoke; milk thistle; hypolipidemic; antioxidant.


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