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Abstract

SERUM ELECTROPHORESIS PATTERN IN BREAST CARCINOMA IN DIFFERENT POPULATIONS IN AN AROUND CHENNAI CITY, TAMIL NADU

Pushpa Rani V.*

ABSTRACT

Serum protein electrophoresis is widely used in clinical laboratories, especially for the detection and identification of paraproteins. Traditional clinical electrophoretic procedures are manual methods that use agarose gels or cellulose acetate membranes as the separation bed. Quantitation of the five major serum fractions is done by densitometric scanning of the gel or the membrane. Clinical interpretation is based on the alteration of the content of one or more of the five fractions. Agarose as the supporting medium for protein electrophoresis is reported to give better resolution than cellulose acetate, with increased ability to detect paraproteins. Densitometric scanning depicted five serum protein fractions albumin, 1 albumin, 2 albumin,  globulin, and  globulin in breast cancer subjects. The present also reported that ages of the three groups differed (31-40, 41-50, 51-60 yrs), correlation analysis between serum protein levels and the age of the patients failed to reveal any statistically significant relationship. The percentage of the different serum protein fractions obtained was in the normal range like the control.

Keywords: Serum protein Electrophoresis, Immunoglobulin, Densitometer scanning, Breast cancer.


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