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Abstract

ANTIBACTERIAL EFFECT OF FRACTION AND CRUDE EXTRACT OF TERMINALIA CHEBULA ON STREPTOCOCCUS PNEUMONIA AND STAPHYLOCOCCUS AUREUS

Ganesan S.* and Rajakumar R.

ABSTRACT

This study investigated the effect of Terminalia chebula crude extract and fraction on two pathogenic bacteria. Generally, crude extracts and fractions were effective against both bacterial strains by disc diffusion method, respectively. Nevertheless, ethanol mixes methanol fraction at 8:12 and 10:10 combinations were showed 5 mm and 7 mm zone of inhibition against Streptococcus pneumonia and Staphylococcus aureus, which was better anti-bacterial activity than the all separate crude extract and fractions. Compared to crude extract, the fraction elicited higher antibacterial properties. The major components of extracts tested were identified by gas chromatography coupled with mass spectrometry (GC/MS) analysis. Our result showed that ethanol with methanol fraction extracts of Terminalia chebula can be used alongside conventional antibiotics to contest agents of infections that are so predominant in the clinics.

Keywords: Terminalia chebula, crude extracts, GC-MS analysis and anti-bacterial activity.


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