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Dnyaneshwar Pawar, Priya Mourya*, Omkar Kamble, Prajakta, Kshirsagar and Aniket Patil


The present study of trustworthy processes for the synthesis of Nanosized materials is of countless importance in the field of nanotechnology. Biosynthesis of Silver Nanoparticles (AgNPs) using bacteria has arriving weighty interest because of their potential to synthesize nanoparticles. In the current study, synthesis of silver nanoparticles by a bacterial strain (DA-12) isolated from soil is reported. The bacterium was isolated, screened and characterized by morphological, biochemical and 16S rRNA analyses. Molecular identification of the isolate was done which showed a strain is Bacillus shackletonii DA-12. When treating the isolated bacteria with 1mm Silver nitrate (AgNO3), it was found to have the proficiency to form silver nanoparticles at 370 C within 24 hours of incubation. This was confirmed by the visual observation and FT-IR analysis. Therefore, the current study is a much of an effective synthesis of silver nanoparticle by the present Bacillus strain. Silver nanoparticles were also tested against antibacterial potential of some human pathogens.

Keywords: Bacillus shackletonii DA-12.

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