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Abstract

ISOLATION, SCREENING AND OPTIMIZATION OF NOVEL LACCASE PRODUCTION FROM PENICILLIUM CHRYSOGENUM

Ganiya,R and Anuradha,R

ABSTRACT

The present work focuses on isolating, screening and optimizing the process parameters to achieve the maximum production of extracellular laccases by penicillium chrysogenum obtained from marine soil. Guaiacol was used as substrates for screening the laccase activity. Culture condition like pH, temperature and nutritional parameter like carbon source were optimized. The optimization studies revealed that the laccase yield was highest when operated at pH 6.0 tempherature 60◦C and carbon source-maltose in the production medium. The present study showed that relatively simple plate test screening method can be used for discovery of novel laccases.

Keywords: Laccases, penicillium chrysogenum, isolation, Guaiacol, screening.


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