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Abstract

RPHPLC-DAD METHOD FOR SIMULTANEOUS ESTIMATION OF CEFIXIME TRIHYDRATE AND OFLOXACIN IN COMBINED TABLET DOSAGE FORM

Mangesh H. Hingankar, Pravin K. Bhoyar, Sachin B. Dudhe

ABSTRACT

A simple, accurate, sensitive and validated RP‐HPLC method involved with Diode Array Detection was developed for simultaneous determination of Cefiximetrihydrateand Ofloxacin in combined tablet dosage form has been developed. The sample was analyzed by reverse phase Teknokroma (Kromosil) C8 (150 x 0.6 mm i.d.) column as stationary phase with PDA detection at 285 nm. The separation was carried out using a mobile phase consisting of 0.01 %v/v Triethylaminephosphate buffer of pH 5.2 and Acetonitrile in the ratio of 80: 20 at a flow rate of 0.8 ml/min. at ambient temperature conditions. The method was validated for Accuracy, Precision, Linearity, Ruggedness, and Robustness. The method was successfully applied on tablet dosage form.

Keywords: RP?HPLC, Cefixime trihydrate, Ofloxacin, Tablet dosage form.


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