Abstract

DECOLORIZATION POTENTIAL OF BACILLUS SPECIES FOR REMOVAL OF SYNTHETIC DYES SUCH AS MALACHITE GREEN AND METHYLENE BLUE

Vemula Vani*, Deepak K. Naik, Leelamani B. and Syed Faraz

Abstract

Bacteria have potential ability to decolorize synthetic commercial dyes used for textile dyeing. Effluents from textile and dyeing industries cause serious pollution of soil, water and environment. Therefore, this study was aimed to screen for the bacteria capable of decolorizing Malachite green and Methylene blue. The decolorization potential for two textile dyes by the Bacillus species isolated from dye contaminated soil of a local dying industry near Bangalore was determined. Different parameters such as pH, time and temperature were optimized for the present study. The optimum pH, temperature and incubation time were found to be pH 7, 45°C and 72 hours respectively. It was found that Bacillus species decolorize 92% of Malachite green (100mg/L) and 69% of Methylene blue (100mg/L) at 45°C after 72 hours under shaking conditions. As these Bacillus species have the ability to decolorize the textile dyes, these bacterial strains could be used to degrade different dyes of textile industry.

Keywords: Dye decolorization, Malachite green, Methylene blue, and Bacillus species.