Abstract

IMMUNOHISTOCHEMICAL STUDY IN COMPARISON OF ONCOGENE HER1 AND HER2 WITH HORMONAL EXPRESSION IN PRIMARY IRAQI BREAST CANCER

Ali Hussein Al-Khafaji, Sahar A. H. Al-Sharqi* and  Ekhlass Noori Ali

Abstract

Breast cancer is a leading cause of cancer-related deaths in women worldwide.The objective of this current project was to expression the HER1 (Human epidermal growth factor receptor 1), HER2 (Human epidermal growth factor receptor 2), ER (Estrogen receptor), PR (Progesterone receptor), Ki67 and CK18 (Cytokeratin 18) among primary breast cancers. The expression of HER1, HER2, ER, PR, Ki- 67, and CK18 was studied by immunohisto chemical (IHC) analysis in 50 patients breast cancer. The molecular classification was used in commonly breastcancer by IHC analysis. Based on this analysis, there were characterized five molecularly different subclasses: luminal type A and B, HER-2, basal-like and unclassified. In multivariate analysis, weexamined the significance of HER1 with other biomarkers which was showed HER1 significant difference with HER2, ER and Ki67.In addition, Luminal A subtype presented a higher percentage (38%) than other molecular subtypes but Luminal B and unclassified subtypes recorded a lower percentage (12%).HER1 a significant difference (p≤0.05) association with TNM(Tumor size, Lymph Node status, Metastases) stage (I+II) of the patients was showed. Also, we found a significant difference(p≤0.05) association between HER2 with age ≥50 and TNM stage (I+II). Moreover,significant difference (p≤0.05) association was noticedbetween both ER and CK18 with invasive ductal carcinoma.Our findings suggest that HER1 plays an important role in the pathogenesis of primary breast cancer and associated with clinicopathological parameters and molecular subtypes.

Keywords: Breast cancer,HER1,immunohistochemistry,molecular classification.