Abstract

IN VITRO CLONING OF SIMMONDSIA CHINENSIS (LINK ) SCHNI USING BAP AND KINETIN

Sudhanshu^a, Shakera Khan^a, Ekta Menghani^b* and Mohit Soni^b

^aSuresh Gyan Vihar University Mahal, Jagatpura, Jaipur, India
^bMahatma Gandhi Institute of Applied Sciences, JECRC University, Jaipur, India

Abstract

The study was conducted to initiate shoots from in vitro, from 5 year old plants of Jojoba. MS medium was supplemented with cytokines i.e., BAP and kinetin each at the rate of 0.5, 1.0, 2.0, 3.0 (mg / L) in the are experiment and at the rate of 0.5, 1.0, 2.0, 3.0 mg/L in the other experiment. In the 1st experiment, the lowest concentration gave slow response. The shoots differentiated from thick explants were healthier and these grow more vigorously, at 28 + 20C on MS medium supplemented with 2.0 mg/L BAP. In the 2nd experiment kinetin was found to be less effective cytokines than BAP for shoot induction. The shoot bud differentiation was positively affected by incorporation of additives in medium. If the additive were not added, yellowing of shoots occurred.

Keywords: Jojoba, Simmondsia chinensis, Shoots, BAP - 6 – benzylaminopurime, Kinetin.