Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CLOTRIMAZOLE AND METRONIDAZOLE IN FORMULATION BY RP-HPLC METHOD

Hardha B.*, P. Devi and V. Sekar

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Abstract

A Reverse Phase High pressure liquid chromatographic method has been developed for simultaneous estimation of Mertonidazole and Clotrimaole in its Soft gelatin Capsule form. Acolumn C8-250 mm x4.6 mm, 5μm in isocratic mode, with mobile phase A 80 VOLUMES OF Buffer and 20 volumes of Methanol mixed well. The flow rate was 1.0ml per minute and effluent was monitored at 210 nm. The approximate retention time for Metronidazole and Clotrimazole were 7.16 min and 17.36 min respectively. The linearity for Metronidazole and Clotrimazole was in the range of 50% to 150% respectively. The proposed method is precise and rapid for simultaneous estimation of Metronidazole and clotrimazole in Soft gelatin Capsule formulation.

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