Abstract

INVESTIGATION OF CYTOGENETIC EFFECT OF ORANGE COMMINUTED IN THE CULTURED HUMAN PERIPHERAL BLOOD LYMPHOCYTES

Ekhlas. M.F. Al -Tai?

Abstract

Orange comminuted, an orange derived paste which can be obtained by mixing several of its components, where juice is the major contributor at this product. It can be used as a natural basis to enhance sensory properties of soft drinks, despite the importance of this product. There are not available studies illustrate precisely the cytogenetic effects of this product. Therefore, in the present study, analyzed the comminuted spectrophotometrically, free and total phenolics, analyzed by Folin-Ciocalteu colorimetric method, compared with orange juice, then determined the cytogenetic effects of concentrations comminuted orange (5,10,15) μg / mL and the control (did not receive any additive) in cultured peripheral blood lymphocytes (in vitro), using cytogenetics parameters mitotic index(MI), replication index (RI), and micronucleus (MN). Results showed that the concentrations used did not induce an increase in the frequency of micronucleus (MN), replication index (RI) and mitotic index(MI). The results came, almost similar to that of control which indicates that the orange comminuted product, has no significant cytotoxicity and genotoxicity effects in vitro on human lymphocytes. Thus, it could be concluded that comminuted orange polyphenols protect the normal cells from genotoxic and carcinogenic agents, which indicates the therapeutic antioxidative role of flavonoids, or other orange comminuted compounds.

Keywords: Orange comminuted, Phenolic compounds, Cytogenetic, peripheral lymphocytes, Mitotic index, Replication index, Micronucleus.