Abstract

EVALUATION OF MONO SODIUM GLUTAMATE INDUCED HEPATOTOXICITY IN ADULT WISTAR ALBINO RATS

Sandharbh Kumar*, Nitesh Kumar and Bhoopendra Kumar

Abstract

The main significance of this research is to investigate the effect Mono Sodium Glutamate (MSG) on the liver of Wistar albino rats on a daily intake for a different time interval. Forty Wistar albino rats of body weight 200±30 gm (6-7 month old) were used in the present study. All the animals acclimatized for one week in standard laboratory condition. After acclimatization all the animals were divided into four groups of five male and five females i.e. one control and three treatment groups. Group I served as a control and received normal distilled water while group II, III and IV received MSG at the dose level of 70 mg/100 gm of body weight daily for a period of 30 days, 45 days and 60 days, respectively. At the end of the experimentation, all the animals were sacrificed and the liver was removed, weighed and processed for histopathological examination. The blood sample was also collected from rats on day 31st for group I and II, day 46th for group III and day 61st for group IV from the direct heart puncture of each animal in sterilized EDTA and heparin vial for haematological and biochemical observation. The Wistar albino rats treated with the MSG showed varying degree of toxicological consequences but these consequences were more severe in that group of animals that received MSG for a long period of time duration. Decreased in body weight observed in that group of animals who received MSG at the dose level of 70 mg/100 gm of body weight for a period of 60 days. However, decrease in Haemoglobin, PCV and Neutrophill was also observed in group III and group IV (MSG at the dose level of 70 mg/100 gm of body weight for a period of 45 days and 60 day). The value of RBC was also decreased in the group IV (MSG at the dose level of 70 mg/100 gm of body weight for a period of 60 days). However the value of WBC was significantly increased in the animal of group III and group IV. In the biochemical parameters, the value of SGPT and SGOT was significantly increased in the group III and group IV (MSG at the dose level of 70 mg/100 gm of body weight for a period of 45 and 60 days). BUN and Creatinine were significantly increased in the group IV animals. Liver histopathology showed loss of liver architecture, cell death, dilation of central vein, highly dialated sinusoidal space with thickened tunica media and pyknotic cells.

Keywords: Hepatotoxicity, Mono Sodium Glutamate, Wistar Albino Rats.