Abstract

MICROPROPAGATION OF ENDEMIC AND ENDANGERED TREE TAXA OFTERMINALIA PALLIDA BRANDIS OF TIRUMALA HILLS

R. Vinolya Kumari*, T. Pullaia and P.Manohar

Abstract

Terminalia pallid Bramdis is narrow endemic endangered and threatened medicinal tree taxa on Tirumala hills of Eastern Ghats. It is one of the oldest medicinal herb of India, is an ingredient of Ayurvedic drug “triphala” used for the treatment of hepatic disorders. This globally threatened plant is over exploited for its medicinal use. The present work is aimed to develop a protocol for micropropogation method for rapid and large scale propagation of the Terminalia pallid by using cotyledonary node explants of In vitro grow seedling. B5 is the most suitable medium for multiplication than WPM and MS medium. 5-6 shoot were on B5 medium containing 2 and 5mg/l BAP + 0.5 mg/l NAA. Rooting was best induced in shoot excised from proliferated shoot culture on MS 1mg/l BAP+2mg/l NAA. The rooted plant was successfully acclimatized in the soil.

Keywords: KN – Kinetin, BAP – N6 – Benzyl Aminopurine ; IAA – Indole 3 – acetic acid; IBA – Indole butyric acid; NAA – 1 - Naphthalene acetic acid.