Abstract

ANTITHROMBOTIC, CYTOTOXIC AND ANTIBACTERIAL ACTIVITIES OF METHANOL EXTRACT OF FICUS SAGITTATA (VAHL) LEAVES.

Mahmudul Hasan*, Mohammad Shah Hafez Kabir, Syed Md. Abdul Kader, Md. Saif Uddin, Md. Abu Aiube Ansary, Mohammad Zia Habib, Atiqur Rahman, Md. Azahar Uddin, Md. Ismail Hossain, Abul Hasanat and Md. Rabiul Islam

Abstract

Extract from the leaves of Ficus sagittata (Vahl) were screened for their antithrombotic, cytotoxic and antimicrobial exercises. The cytotoxicity was surveyed with the brine shrimp lethality bioassay and antithrombotic impact with human blood by clot lysis method. The brine shrimp lethality bioassay was utilized to assess cytotoxicity (LC50 = 226.85 μg/ml) contrasted with Vincristine sulfate (LC50 = 0.74 μg/ml). It was also assessed as antithrombotic activity when contrasted with streptokinase. It has significant antithrombotic movement (56.13±2.72%) contrasted with standard streptokinase (81.32±1.46%). The extract indicated zone of inhibition against Gram positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram negative bacteria (Salmonella typhi, Salmonella paratyphi, Escherichia coli, Pseudomonas aeruginosa) at 1000 μg/disk. Gram positive bacteria Bacillus subtilis demonstrated no action against F. sagittata leaves extract. Relative percentage inhibition of the extract against each bacterium also calculated. These results indicate that F. sagittata have favorable antithrombotic, cytotoxic and antibacterial effects and capacities of F. sagittata extract to be processed for pharmaceutical use.

Keywords: Ficus sagittata, Antithrombotic, cytotoxic, antibacterial.