Abstract

FOOD SUPPLEMENTS WITH ANABOLIC AND ANDROGENIC ACTIVITY - UHPLC ANALYSIS OF FOOD ADDITIVES, CONTAINING TRIBULUS TERRESTRIS EXTRACT.

S. Ivanova, K. Ivanov, R. Mladenov, S. Papanov, Stef. Ivanova, D. Obreshkova,
??tar Atanasov and V. Petkova*

Abstract

Over the last 50 years AAS have been used by many sportsmen to improve physical endurance and better results in sport. The widespread use of AAS is a problem because of the serious side effects of these medicines. Long-term effects affect the cardiovascular system, mental health, endocrine system. Infertility defects, feminization and masculinization are often irreversible side effects. Rate risk - benefit shows that any kind of AAS abuse is extremely dangerous. AAS can be used legally only like prescribed medicines and only for medical purposes. Many athletes prefer taking food supplements with androgenic effects instead of conventional steroids because of their safety profile. By the end of 2004 Prohormones have been one of the most attractive supplements with anabolic and androgenic effects. In 2005 the use and distribution of the majority of these Prohormones became illegal. Nowadays there is a big interest about food additives, containing extract of Tribulus Terrestris because of their androgenic and anabolic activity. In this work we have demonstrated a UHPLC method of analysis of FA and OTC, containing extract of Tribulus Terrestris with mass spectral detection with high resolution (HRMS) and atmospheric pressure ionization "electrospray". This combination gives as quickly and as efficiently split and the possibility of identifying the separated components of the samples analyzed by the exact weight of their molecular ions.

Keywords: anabolic androgenic steroid; Tribulus, Tribulus terrestris, steroids; side effects, sport medicine , food additives, androgenic, anabolic, UHPLC, saponins, saponin