Abstract

ESTIMATION OF PROTEIN CONCENTRATION IN CELL LINES AFTER TREATED WITH TOXIN A EXTRACTED FROM PSEUDOMONAS AERUGINOSA

Bushra Hindi Saleh*

Abstract

Two cell lines REF (Primary Rat Embryo Fibroblast) and AMN3 (Ahmed-Mohammed-Nahi-2003) were used to investigated the role of toxin A extracted from pseudomonas aeruginosa in inhibition of protein synthesis in cell lines after exposure to purified toxin A. Results have shown that incubation of toxin A (purified toxin A alone in first group and purified toxin A activated with urea in the second group) at the concentration (5, 25, 50, 100) ng /ml with both REF and AMN3 cell lines for 48 hrs will cause significant decrease in protein concentration in both cell lines in comparison with untreated control when estimated by Bradford method.

Keywords: pseudomonas aeruginosa, purified toxin.