Abstract

MOLECULAR STUDY FOR SOME VIRULENCE FACTORS OF ACINETOBACTER BAUMANNII ISOLATED FROM PATIENTS WITH WOUND INFECTION IN HILLA CITY

*Zahraa Abd AL Mahdi, Ilham Abass Bunyan and Maysaa Salih AL Shukri

Abstract

This study is devoted to the isolation and identification of A.baumannii; indeed seven isolates of A.baumannii have been isolated and identified out of 100wound swab collect from 100 patients with wound infection, The isolates obtained have been cultured in selective media and biochemical identified. Besides, some virulence factors have been detected by such molecular techniques as polymerase chain reaction PCR and it has been found that only 2(28%) of isolates gave positive result for cnf1 at 498bp, 4(57.1%) of them gave positive result for kps at 272bp, 2(28.5%) of them gave positive result for PAI at 930bp, 5(71.4%) of them gave positive result for ibeA at 170bp, 1(14.2%) of them gave positive result for sfa at 240bp, 4(57.1%) of them gave positive result for iutA at 300bp.

Keywords: A.baumannii, cnf1, kps, ibeA.