Abstract

ULTRA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC): A NEW TREND IN ANALYSIS

Dr. Sayyed Hussain and Tabrez Shaikh*

Abstract

UPLC is an advance technique of liquid chromatography where it takes advantage of innovation in various technologies such as instrumentation and particle size to achieve dramatic increases in resolution, speed and sensitivity of the liquid chromatography. It operates at higher pressure than that used in HPLC and uses fine particles (less than 2.5μm) & mobile phases at high linear velocities. UPLC Technology is now applied throughout the world produce quality data with reproducible and robust methods as compared to the conventional HPLC. UPLC can be hyphenated with other techniques such as Mass spectrometer (MS), Ion chromatograph (IC), Nuclear magnetic resonance spectrometer (NMR) and Infrared spectrometer (IR) etc. This technique provides unique end-to-end solutions for all industries and has found application in various fields such as pharmaceutical, food, environmental, forensic, toxicology and pesticide.

Keywords: UPLC, Theory, Detectors, Advantage, Applications.