Abstract

BIOSYNTHESIS OF SILVER NANOPARTICLES BY MELIA DUBIA LEAF AQUEOUS EXTRACT AND ITS ANTIBACTERIAL ACTIVITY

J. Rosaline Vimala*, S. Margrat Sheela, G. Dayana Jeya Leela and S. Irudaya Monisha

Abstract

Nanotechnology is an emerging field in the area of interdisciplinary research, especially in biotechnology. In this study the silver nanoparticles were synthesized by using a plant leaf extract of Melia dubia. The synthesized silver nanoparticles were confirmed by the change of colour after addition of leaf extract into the silver nitrate solution. The biosynthesized AgNPs were characterized by using UV-Vis analysis, Fourier Transform Infrared analysis (FTIR), X-ray diffraction analysis (XRD) and Scanning Electron Microscopy (SEM analysis. Antimicrobial activity of the silver bio-nanoparticles was performed by well diffusion method against Vibrio cholerae, Escherichia coli, staphylococcus aureus and Klebsiella pneumonia. The highest antimicrobial activity of silver nanoparticles synthesized by Melia dubia extract was found against Vibrio cholerae (22 mm) and E. coli (22 mm) respectively. The Ag NPs synthesized in this process has the efficient antimicrobial activity against pathogenic bacteria.

Keywords: Green synthesis, Silver nanoparticles, FT-IR, SEM, XRD, Antimicrobial property.