Abstract

PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF POLYHERBAL FORMULATION FOR ANTI ACNE ACTIVITY

Vishnu Raj*, Ashok Peepliwal and S. K. Lariya

Abstract

The present study was undertaken anti-bacterial and anti-oxidant activity of methanol extract of Curcuma longa, Bombax ceiba, Ficus bengalensis, Lens culinaris. The antibacterial activity, total phenolics and flavinoids were determined by agar-well diffusion method. The zones of inhibition of methanol extracts of Curcuma longa, Bombax ceiba, Ficus bengalensis and Lens culinaris were found to be 13.375, 11.35, 9.725, 8.625 mm respectively the DMSO did not show any significant antibacterial activity. Anti-oxidant potential was evaluated by using in vitro methods such as 1,1- diphenyl-2- picrylhydrazyl (DPPH), nitro blue tetrazolium chloride test (NBT), OH-, and O2 - inhibition free radical assay. The absorbance was measured in UV/vis. Spectroscopy at 590. The total phenolic content (TPC) was expressed as mg/gm of gallic acid equivalent of dry extract sample in order Bombax ceiba 27.56 mg/gm > Ficus bengalensi 15.65 mg/gm,> Curcuma longa 11.27 mg/gm, > Lens culinaris 9.68 mg/gm. Total flavonoids content (TFC) was expressed as mg/gm of quercetin in order Curcuma longa 21.23 mg/gm> Bombax ceiba 18.56 mg/gm> Ficus bengalensi 16.56 mg/gm> Lens culinaris 12.56 mg/gm. The different fractions significantly (p < 0.05) reduce DPPH radical in a dose dependent manner at a concentration for inhibition effect of methanol extract. Suitable ploy herbal formulation is prepared using combination of effacious bioactive and evaluated for anti acne activity.

Keywords: P. acnes, anti-bacterial, anti-oxidant, total phenolic content, total flavonoids content, UV- absorption spectra, gel.