Abstract

PHYTOCHEMICAL ANALYSIS & IN VITRO ANTICANCER ACTIVITY OF METHANOL EXTRACT OF DECALEPIS HAMILTONII ROOT AGAINST HEPATIC CANCER CELL LINES (HepG2)

Devi Manickam* and Preetha D.

Abstract

Hepatocellular carcinoma is the third leading cancer causing death Worldwide. Currently research focuses on the use of medicinal plants for the treatment of cancer, due to its resistance to chemotherapeutic agents. The present study was designed to evaluate the preliminary phytochemical analysis and in vitro anticancer activity of methanol extract of Decalepis hamiltonii root against hepatic cancer cells (HepG2). Various concentrations (125, 250, 500, 750 & 1000μg/ml) of methanol extract of Decalepis hamiltonii was taken for cytotoxicity assay using MTT assay and calculated the percentage of cell viability. The phytochemical analysis revealed the presence of flavonoids, saponins, tannins, steroids, cardiac glycosides, phenols, resins and thiols. The methanol extract of Decalepis hamiltonii showed therapeutic values against HepG2 cell line with CTC50 values 476.67±1.3 respectively. 1000μg/ml of Decalepis hamiltonii has maximum (76.86±1.4) cytotoxicity effect against HepG2. The result of our study revealed that the methanol extract of root of Decalepis hamiltonii has a cytotoxic effect on HepG2 cell line in a concentration dependent manner. The isolation of pure compounds and determination of the bioactivity of individual compounds will be further performed.

Keywords: Decalepis hamiltonii, Phytochemical analysis, In vitro anticancer activity, Hepatic cancer cells (HepG2).