Abstract

ISOLATION, IDENTIFICATION & ANTIBIOTIC SENSITIVITY OF BACTERIA OBTAINED FROM COMPARATIVE MICROBIAL ANALYSIS OF REFRIGERATED AND NON-REFRIGERATED FRUIT CONTAINING SOFT DRINKS FROM CHENNAI, TAMIL NADU, INDIA.

V. Pushpa Rani*, E. Saravanan, A. Anitha Nancy, K. Shanmuga Priya and
G. Meena Kumari

Abstract

The present study deals with comparative analysis between refrigerated and non-refrigerated fruit containing soft drinks for quantitative microbial analysis. The number of individual colonies on an agar plate was counted using by Colony Counter for both refrigerated and nonrefrigerated. Based on the Morphological and Cultural characteristics, the bacteria was identified and isolated. The Bacterial isolates were analysed for Gram staining characterization and their motility and various biochemical tests such as Indole Test, Methyl Red Test, Triple Sugar Iron Agar and Citrate Utilization Test were performed by serial dilution of well-isolated colony from soft drinks. Based on biochemical analysis (IMViC), the bacteria isolated were found to be E.coli, Staphylococcus, Proteus sp, Pseudomonas sp and Bacillus from the refrigerated and non-refrigerated fruit containing soft drinks. The current investigation was extended to determine the antibiotic sensitivity whether the tested bacterial species is sensitive or resistant to the tested antibiotics. The bacteria which present in the fruit containing soft drinks were more sensitive and few shows resistance towards the antibiotics such as Ciprofloxacin, Amoxicillin Clavulanate, Tetracycline, Penicillin-G and Azithromycin.

Keywords: E.coli, Staphylococcus, Proteus sp, Pseudomonas sp and Bacillus.