Abstract

EVALUATION ON DEGREE OF BIODEGRADATION OF AZO DYE BY EXTRA-CELLULAR AND INTRACELLULAR ENZYMES FROM HALOTOLERANT BACTERIAL STRAIN.

V. Pushpa Rani, K. Shanmuga Priya, A. Anitha Nancy, G. Meena Kumari and
Lousi M.R. Lima Mirabel

Abstract

Numerous biotechnological approaches have been suggested to overcome the problem of physiochemical treatment methods using microorganisms for the treatment of textile dyes and industry effluent as microorganisms play crucial roles in the mineralization of xenobiotic compounds. In this present study halotolerant bacterial strains were isolated and the identification process of halotolerant strain Bacillus flexus was selected on the basis on adaptation of identified strains over different NaCl concentrations and the time required for the formation of well-developed colonies under extreme saline conditions. Enzymatic degradation shows higher potential in degradation of azo dyes. Acid Blue 113 biodegradation by B. flexus at 25 ppm, 50 ppm and 100 ppm shows higher degree of degradation by Extra-cellular enzyme at all interval times (0th, 16th, 24th, 40th, 48th, 64th and 72nd hours) than Intra-cellular enzymes. Intra-cellular enzymes degradation activity was greater in the time intervals of 0th, 16th, 24th and 40th hrs. From the time interval of 48th, 64th and 72nd hours Extra-cellular enzyme predominately degraded Acid Blue 113.

Keywords: Bacillus flexus, halotolerant bacteria, azo dye, Acid Blue 113, and biodegradation.