Abstract

INVESTIGATION OF ENZYMATIC DEGRADATION OF ACID BLUE 113 BY HALOTOLERANT STRAIN PSEUDOMONAS AERUGINOSA

V. Pushpa Rani*, K. Shanmuga Priya, A. Anitha Nancy3 and G. Meena Kumari

Abstract

The azo dyes are some of the most commonly used in the leather and textile industries as they are quite versatile in nature. Microbial degradation of azo dyes is mediated by enzymes. In this present study an attempt primed for the evaluation on degree of biodegradation of Acid Blue 113 azo dye by extra-cellular and intracellular enzymes from halotolerant bacterial strain. Preceding studies on the enzymatic biodegradation of azo dyes werenot distinguished extra-cellular and intra-cellular enzymes. The results found in this study shows that Extra-cellular enzymes exhibits higher degree of degradation than Intra-cellular enzymes at different interval of time (0th, 16th, 24th, 40th, 48th, 64th and 72nd hours) with varies dye concentration (25ppm, 50ppm, 75ppm &100ppm).

Keywords: Biodegradation, halotolerant bacteria, Acid Blue 113, Extra-cellular enzyme, Intra-cellular enzyme and Pseudomonas aeruginosa.