Abstract

PHYTOCHEMICAL COMPOSITION AND CYTOTOXIC POTENTIAL OF EDIBLE RATTAN (CALAMUS TENUIS ROXB.) SHOOT EXTRACTS ON MCF7 AND A549 CELLS.

Pawan Kumar Thakur*, Mini Sheth, Gagandeep Kaur Bhambra, Padamnabhi S Nagar, Kapil Upadhyay, Ranjitsinh Devkar

Abstract

Objective: This study assesses phytoconstituents of Calamus tenuis Roxb. (CT) and further studies its cytotoxic potential in two carcinoma cells lines. Method: Phytochemical extraction was done using hexane, ethyl acetate and methanol as extraction solvents. Cytotoxic potential was assessed via MTT assay against A549 and MCF-7 cell lines. Results: Hexane extract was rich in saponins and steroids whereas, ethyl acetate extract recorded presence of steroids only. These fractions were not able to induce any cytotoxic effect on the said cell lines. However, methanolic extract containing carbohydrates, saponins, flavonoids, steroids, tannins and glycosides accounted for significant cytotoxic activity. Conclusion: This study is the first to investigate the phytoconstituents of Calamus tenuis Roxb. shoot and its subsequent cytotoxicity on carcinoma cells that implies towards its anticancer potential.

Keywords: Calamus tenuis Roxb., phytoconstituents, cytotoxicity, MTT, A549 cells, MCF-7 cells.