Abstract

CYTOTOXIC EVALUATION OF ALCHEMILLA VULGARIS EXTRACT IN NORMAL BLOOD LYMPHOCYTES

Saif L. Al-Osaj* and Khulood W. Al-Sammarraei

Abstract

Objective: The main aim of this study is to investigate the cytotoxic effect of the Alchemilla vulgaris estimated flavonoids on lymphocytes by MTT assay. Methodology: The aerial parts of plants were dried and extracted in 80% methanol by maceration. Chemical detection of crud plant extracts was performed. The total flavonoids were isolated, subjected to thin layer chromatography (TLC) using different mobile systems. The purified material was read by using HPLC. Extracted A.vulgaris goes throw quantitative measurement to calculate the total flavonoids in the extract. A.vulgaris flavonoids contain rutin as major, quareciten and kaempferol, all of them were investigated qualitatively and quantitatively in the present study. The immunomodulation occur by treated extracted flavonoids with Lymphocytes isolated fromhealthy people to measure the cytotoxic effect of extracted Flavonoids on normal lymphocytes by using MTT dye. The cytotoxic evaluation was read by using sandwich ELISA technique at 450nm. Results: This study indicate that the treatment of the isolated flavonoids with normal lymphocytes show significant effect by suppressing the lymphocytes proliferation with the decrease in the extracted flavonoids concentration as compared with control (untreated lymphocytes in suitable media). Conclusion: Immunomodulation study pointed that A.vulgaris flavonoids suppress the normal lymphocytes proliferation using MTT assay. Recommendation: Further studies with purified phytoconstituents of A.vulgaris extract are needed to comprehend the complete mechanism of Immunomodulation.

Keywords: Alchemilla vulgaris; Immunomodulation; Flavonoids; MTT assay.