Abstract

BETA-SITOSTEROL STIMULATE PHAGOCYTOSIS IN HEPG2 CELL LINE TRANSFECTED WITH HEPATITIS B SURFACE ANTIGEN

Ammar M. Lubeiny*, Wuyun Li, Xu Lin and Elshaikh A. Elobied

Abstract

Hepatocellular carcinoma (HCC) has a range of risk factors and Hepatitis B Virus infection considers as one of these factors which lead to develop HCC. This type of carcinoma is still classified as a complex disease due to many risk factors and the patients with HCC diagnosed always at advance stage. The surgery and transplantation are still the most effective treatment while the response to chemotherapy and radiotherapy has poor effect. Our aim is to study the effect of β-Sitosterol, which is found in many plants on HBsAg and on hepatocellular carcinoma. To address this issue, Huh7, HepG2.2.15( harbouring four copies of HBV DNA ) and HepG2 cell lines were used. The Cell viability assay, Enzyme – Linked Immunosorbent Assay, and Gene Expression were used to evaluate the effect of β- Sitosterol. The result of Gene Ontology shows it is effect on HepG2-HBsAg by stimulating the phagocytosis we conclude β-Sitosterol has a positive regulation of phagocytosis to HepG2 Transfected with HBsAg this process could be help full to eliminate the dead cells caused by the apoptotic effect of β-Sitosterol on hepatocellular carcinoma cell infected with hepatitis B virus.

Keywords: Beta- sitosterol, phagocytosis, HCC, Hepatitis B virus.