Abstract

DEVELOPMENT AND VALIDATION OF RP HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ATENOLOL AND CHLORTHALIDONE IN TABLET FORMULATION

Hitesh C. Pimpale, Parag T. Bakshe*, Sachin S. Rane, Prof. (Dr.) Rajesh Y. Chaudhari, Prof. (Dr.) Vijay R. Patil

Abstract

A new simple, rapid, precise and accurate assay method was developed for simultaneous estimation of Atenolol and Chlorthalidone in pure and tablet formulation. The analytes were separated by RP HPLC on a RP-Purosnosphere C18 column (5 μm, 4.6mm* 250 mm). The mobile phase was methanol: water (60:40 v/v) at 1.1 ml/min flow rate satisfactorily resolve the tertiary mixture. The UV detector was operated at 238 nm for the determination of all the drugs. Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 10-160 μg/ml for Atenolol and 5-25 μg/ml for Chlorthalidone with a R2 0.9957 and 0.9965values respectively. The optimized methods proved to be specific, robust and accurate for the quality control of drugs in bulk drug and pharmaceutical formulations.

Keywords: Atenolol, Chlorthalidone, Method Validation, RP HPLC, ICH, C18.