Abstract

IN-VITRO EVALUATION OF ANTIDIABETIC ACTIVITY ON METHANOL AND PETROLEUM ETHER EXTRACT OF IPOMOEA SEPIARIA LEAVES

Pavan Kumar.Chadalawada*, Manohar Babu.P, S.CH.S.K.R. Nandan.Paruchuri, Rajitha.B, Victor Immanuel.P and B. Pooja

Abstract

Preparation of plant extract was done, the collected fresh leaves were thoroughly cleaned with distilled water, dried well and powdered. It was soaked in absolute ethanol in cold (72 hrs). After three days, the extract was filtered, and then it was evaporated at 400C in cylindrical water bath for the elimination of solvent. A semisolid extract (40g) was obtained after complete elimination of alcohol under reduced pressure. It was stored in refrigerator until used. Evaluate the methanol and petroleum ether extract of Ipomoea sepiaria leaves from Alphaamylase activity through in-vitro method by hydrolysis of starch in presence of α-amylase enzyme. This process was quantified by using iodine, which gives blue colour with starch. Acarbose showed the best when compared to extracts, among all concentration of extracts, the methanol extract of 500mg/ml showed the superior anti-diabetic activity when compared to rest of the concentrations of methanol extracts and petroleum ether extracts. RESULT: Acarbose showed the best anti-diabetic activity when compared to all concentration of extracts, followed by the methanolic extract of 500mg/ml showed less significant anti-diabetic activity. CONCLUSION: From the Ipomoea sepiaria leaves extract finally find out the antidiabetic activity at the dose of 500mg/ml when compared to Acarbose.

Keywords: Diabetes mellitus (DM), anti-diabetic, Acarbose, alpha-amylase inhibition method.