Abstract

DEVELOPMENT AND VALIDATION SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF ZONISAMIDE AND BUPROPION IN SYNTHETIC MIXTURE

Rinal B. Patel* and Paresh U. Patel

Abstract

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of Zonisamide and Bupropion in synthetic mixture. The method is based on the simultaneous equations for analysis of both the drugs using methanol as solvent. Zonisamide has absorbance maxima at 238.5 nm and Bupropion has absorbance maxima at 246 nm in methanol. The linearity was obtained in the concentration range of 4-28 μg/ml and 4-28 μg/ml for Zonisamide and Bupropion, respectively. The concentrations of the drugs were determined by solving simultaneous equations at both the wavelengths. The mean recovery was 99.68 ± 1.06 and 98.96 ± 0.59 for Zonisamide and Bupropion, respectively. The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of Zonisamide and Bupropion in synthetic mixture. The results of analysis have been validated statistically and by recovery studies.

Keywords: Zonisamide, Bupropion, Recovery, Simultaneous equations method, Validation.