Abstract

EXPLORATION OF ACTINOBACTERIA FROM MANGROVE ECOSYSTEM OF KRISHNA DISTRICT, ANDHRA PRADESH

Krishna Naragani*

Abstract

Total 120 actinobacterial strains isolated from the coastal region of Krishna district by using six different pretreatment techniques and two different selective media. High actinobacterial count was found in samples pretreated with calcium carbonate as compared to other pretreatments. The influence of season on the incidence of actinobacteria was investigated. The actinobacterial population in the mangrove ecosystems exhibited common seasonal variation. The highest number of actinobacteria was observed in Rainy season while the counts were minimum in Summer. High actinobacterial count was found on the Starch-casein agar medium (ISP-6), when compared to Yeast extract malt extract and dextrose agar medium (ISP-2). The santimicrobial efficacy of the strains was evaluated by using four solvents such as chloroform, ethyl acetate, methanol and acetone. Among the solvents used, ethyl acetate extract exhibited maximum antimicrobial activity where as the other solvent extracts showed moderate to minimum activity against the test microorganisms.

Keywords: Mangrove Actinobacteria, Seasonal enumeration, antimicrobial activity.