Abstract

IN VITRO ANTIBACTERIAL EFFICIENCY OF KANAMYCIN SULFATE LOADED CHITOSAN NANO PARTICLES AGAINST SELECTED BACTERIAL STRAINS

Mohammed M. Safhi*

Abstract

Bacterial resistance to antibiotics has been attempted to be resolved by various researchers by discovering new antibiotics and chemically modifying existing antimicrobial drugs. Unfortunately, there is no assurance that newly developed antibiotics will not develop resistance. In connection to this, nanotechnology is the most unique technology that can provide suitable tools for the effective targeted delivery of drugs into specific cells. The aim of my study was to formulate and optimize chitosan nanoparticles for Kanamycin sulfate delivery to improve bioavailability and prevent drug resistance. In this work Kanamycin is the drug of choice for demonstrating the efficacy of chitosan nanoparticles against bacterial resistance. The ionic gelation technique was used to formulate kanamycin sulfate to be encapsulated in chitosan nanoparticles. The anti-bacterial efficacy of kanamycin loaded chitosan nanoparticles was evaluated against selected bacterial strains, namely Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Escherichia coli and Klebsiella planticola of clinical origin. In the present study, the maximum activity was found in Escherichia coli followed by Klebsiella planticola, Bacillus subtilis, Streptococcus pyogenes and Staphylococcus aureus. The study suggested that the Kanamycin sulfate showed better activity than the conventional Kanamycin sulfate.

Keywords: Kanamycin sulfate; Chitosan, Nanoparticles; Bacterial resistance.