Abstract

ISOLATION AND IDENTIFICATION OF SOIL BACTERIAL DIVERSITY IN FIRE WORKS AREA

Fidha K.*, Prabhu V.

Abstract

The microbial diversity of soil and water samples collected from areas exposed to pyrotechnical chemicals in Sivakasi, Virudhunagar district (Tamil Nadu, India) was studied. Samples from cultivable land, waste areas, and urban locations within the same region were also analyzed for comparative purposes. The generic composition of the samples from the pyrotechnical exposure sites showed significant differences compared to the other samples. Results from 16S rDNA molecular sequencing indicated that Pseudomonas species were predominant in both the soil and water samples from the pyrotechnically exposed areas, followed by Achromobacter species in the soil and Bacillus and Micrococcus species (with Micrococcus accounting for 16.6%) in the water samples. Notably, Corynebacterium and Micrococcus were completely absent in the pyrochemical-exposed soil, and Achromobacter was missing in the exposed water samples, despite their presence in the other locations tested. This study clearly demonstrates that pollutants from pyrotechnical practices can significantly impact microbial biodiversity. It underscores the need for appropriate measures to control pollution levels and protect biodiversity.

Keywords: Bacterial Diversity pyrotechnical chemicals, Pseudomonas, and Achromobacter.