Abstract

DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROSCOPIC METHOD FOR THE ESTIMATION OF THIOCOLCHICOSIDE IN BULK AND PHARMACEUTICAL DOSAGEFORM

Chakravarthi K. S. S.*, K. B. Ilango, Ananya E. D., Dinesh D., Karthikeyan A., Suwathi P., Rathi R.

Abstract

Thiocolchicoside is a semi-synthetic derivative of colchicoside obtained from the plant Gloriosa superba and is widely used as a centrally acting skeletal muscle relaxant. It possesses musclerelaxant, anti-inflammatory, and analgesic properties and is commonly prescribed for the treatment of painful muscular spasms associated with orthopedic, rheumatologic, and traumatic disorders. The present study was aimed at developing and validating a simple, rapid, precise, and accurate UV spectrophotometric method for the quantitative estimation of Thiocolchicoside in bulk drug and pharmaceutical dosage form. In this study, distilled water was selected as the solvent based on solubility studies. The analysis was performed using a Shimadzu 1900i double beam UV-Visible spectrophotometer with matched quartz cells. The standard drug solution was scanned in the wavelength range of 200–400 nm and the maximum absorbance (λmax) of Thiocolchicoside was found to be 260 nm. A calibration curve was constructed using standard solutions in the concentration range of 2–24 μg/ml, which showed good linearity with a correlation coefficient (R²) of 0.9978 and regression equation of y = 0.0379x + 0.0153. The developed method was validated according to analytical parameters including linearity, precision, accuracy, ruggedness, robustness, limit of detection (LOD), and limit of quantification (LOQ). The LOD and LOQ values were found to be 0.2943 μg/ml and 0.8919 μg/ml respectively. Recovery studies showed percentage recovery within the acceptable range of 98–100.75%, indicating good accuracy of the method. The validated method was successfully applied for the estimation of Thiocolchicoside in a marketed formulation (Myoril 4 mg), and the assay results showed an average drug content of 100.52%. The results indicate that the developed UV spectrophotometric method is simple, economical, accurate, and suitable for routine quality control analysis of Thiocolchicoside in bulk drug and pharmaceutical dosage forms.

Keywords: Thiocolchicoside, UV Spectrophotometry, Method Validation, Pharmaceutical Formulation, Quantitative Analysis.