Abstract

A SIMPLE SPECTROPHOTOMETRIC ASSAY OF TENOFOVIR IN BUILK AND PHARMACEUTICAL FORMULATIONS

K. Prabhavthi, U. Sreenivasulu, J. Sudhakar Reddy and N. Rami Reddy*

Abstract

A simple, sensitive, rapid and accurate spectrophotometric method has been developed for the estimation of Tenofovir in pharmaceutical dosage forms. Tenofovir undergoes diazotisation when treated with sodium nitrite and hydrochloric acid. The excess nitrous acid during the diazotisation is removed by the addition of urea solution. The solution was shaken frequently to allow the nitrogen gas to escape. The diazonium cation reacts with the coupling reagent, resorcinol to produce a orange red azo product. This orange red colour product shows maximum absorbance at 440 nm.

Keywords: Spectrophotometry, resorcinol, Tenofovir, Pharmaceutical and Formulation.