Abstract

IN VIVO AND IN VITRO ANTITUMOR ACTIVITY OF A NOVEL PH ACTIVATED POLYMERIC DRUG DELIVERY SYSTEM FOR DOXORUBICIN

Jyoshna Rani Dash*, Bandana Behera, Debasish Pradhan

Abstract

Introduction: Conventional chemotherapy agent together with Doxorubicin (dox) is of limited clinical use because of its inherently low selectivity, that could lead to systemic toxicity in regular healthy tissue. Methods: A pH stimuli-sensitive conjugate based on polyethylene glycol (PEG) with covalently attachment doxorubicin through hydrazone bond (peg-hyd-dox) changed into organized for tumor focused on delivery gadget. while peg-dox conjugates thru amid bond (peg-ami-dox) turned into synthesized as control. Results: The artificial conjugates were confirmed by using proton nuclear magnetic resonance (nmr) spectroscopy, the release profile of dox from peg-hyd dox turned into acidchargeable for the hydrazone linkage between dox and peg, brought about one-of-a-kind intracellular uptake direction; intracellular accumulation of peg-hyd-dox became higher than peg-ami-dox because of its ph-brought about profile, and thereby more cytotoxicity towards mcf-7, mda-mb-231 (breast most cancers models) and hepg2 (hepatocellular carcinoma model) mobile lines. following the in vitro outcomes, we xenografted mda-mb-231 mobile onto scid mice, peg-hyd-dox confirmed stronger antitumor efficacy than loose dox and became tumor-targeting. Conclusions: effects from those in vivo experiments were regular with our in vitro effects; cautioned this ph-precipitated peg-hyd-dox conjugate may want to target dox to tumor tissues and launch unfastened tablets by means of acidic tumor surroundings, which would be potent in antitumor drug delivery.

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