Abstract

ANTIBACTERIAL POTENTIAL OF THE LEAF EXTRACTS OF AMPELOPTERIS PROLIFERA (RETZ.) COPEL - AN ETHNOMEDICINALLY IMPORTANT FERN IN ANGUL DISTRICT OF ODISHA (INDIA)

Smaranika Nayak*, Ramakanta Mishra, Pradeep Kuamr Chand and
Kunja Bihari Satapathy

Abstract

The objective of the present investigation was to evaluate the antibacterial activity of ethanolic, methanolic and aqueous extracts of Ampelopteris prolifera (Retz.) Copel leaves. The antibacterial potential of the fern was tested against human pathogens such as Staphylococcus aureus (MTCC-1430), Vibrio choleriae (MTCC-3906), Pseudomonas aeruginosa (MTCC-1035), Shigella flexneri (MTCC-9543), Bacillus subtilis (MTCC-1305), Streptococcus mitis (MTCC-2897), Klebsiella pneumoniae (MTCC-109), Staphylococcus aureus (MTCC-1430), Salmonella paratyphi (MTCC-3220), Micrococcus luteus (MTCC- 1809), Salmonella typhimurium (MTCC-98), Escherchia coli (MTCC- 614), Bacillus circulans (MTCC-490) and Enterococcus faccalis (MTCC-459) by following agar well diffusion method. The result of The study revealed that ethanolic extract was highly effective against Streptococcus mitis and Klebsiella pneumoniae while Pseudomonas aeruginosa showed no response and Salmonella enterica ser typhi, Bacillus subtilis, Streptococcus mitis and Staphylococcus aureus responded moderately. The ethanolic extract had high inhibition against Streptococcus mitis (17.16 ± 0.20 mm) whereas Shigella flexneri showed no response and moderate effect against other test bacteria. Streptomycin was taken as the reference antibiotic.

Keywords: Ampelopteris prolifera, Agar well diffusion, Antibacterial activity, Ethanol, Methanol, Streptomycin.