Abstract

ANTIPROLIFERATION ACTIVITY OF OCIMUM GRATISSIMUM AQUEOUS EXTRACT ON HUMAN BREAST CANCER MCF-7 CELL LINE

Shaktiprasad Pradhan*, Debasish Pradhan, Bandana Behera

Abstract

Objective: Ocimum gratissimum (OG) has been used in traditional systems of medicine as it has marked remedial activities against many diseases including cancer. Our study is a part of an ongoing search for potential anticancer agents in the ethnomedicinal plants of Odisha. Methods: Different concentrations of OG aqueous extract were exposed to Human breast cancer MCF-7 cell line at different time intervals. In-vitro cytotoxicity study with evaluation of growth inhibition was done by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide] assay, regardless of ER status. Observation of the changes in cell morphology & detection of apoptosis was done. The various concentrations of the aqueous extract of OG were used & effective dose was calculated from dose-response curve. Results: After treatment an increased rate of cell death was observed in the MCF-7 cells. The antiproliferation activity on MCF-7 cell line was evaluated & found to have significant growth inhibitory effect with IC(50) value 41.7 microg/ml. Conclusion: This proposed that the aqueous extract of OG be appropriate for potential anticancer activity through growth inhibition and apoptosis on human breast cancer cell. Further purification and characterization of the aqueous OG extract might bring more potential sources of bio active molecules.

Keywords: Antiproliferation, Ocimum gratissimum (OG), Aqueous extract, Breast cancer, MCF-7 MTT assay.